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The media of combined lymphocyte and splenocytes cultures of mice containing secreted cytokines were used to detect different cytokine levels. Levels of cytokines were quantified by multi-analyte ELISA. The absorption was measured at 450 nm by the spectrophotometer. Absorbance values more than two times the corresponding negative control absorbance values are interpreted as positive samples according to manufacturers’ instructions and indicated with an asterisks (*). rNP: mice immunized with rNP, NC: mice immunized with PBS as a negative control group.

Journal: PLoS Neglected Tropical Diseases

Article Title: Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)

doi: 10.1371/journal.pntd.0009973

Figure Lengend Snippet: The media of combined lymphocyte and splenocytes cultures of mice containing secreted cytokines were used to detect different cytokine levels. Levels of cytokines were quantified by multi-analyte ELISA. The absorption was measured at 450 nm by the spectrophotometer. Absorbance values more than two times the corresponding negative control absorbance values are interpreted as positive samples according to manufacturers’ instructions and indicated with an asterisks (*). rNP: mice immunized with rNP, NC: mice immunized with PBS as a negative control group.

Article Snippet: The mice lymphocytes and splenocytes were stimulated with rNP as described at LPA and culture supernatants were harvested and analyzed by the Mouse Multi-Analyte ELISA Array (Qiagen, Germantown, MD) containing interleukin (IL)-1B, IL-4, IL-6, IL-10, IL-12, IL-17A, IFN-γ, G-CSF, and GM-CSF according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Spectrophotometry, Negative Control